Abstract
This experiment was suggested to study the beneficial effects of foliar application with propolis extract at the rates 0, 6000, 7000, 8000 and 9000 mg/L solution used as foliar application to spinach seedlings on growth, yield and some chemical constituents of spinach plants (Spinacia oleracea L.) grown under calcareous saline soil conditions. All experiments were achieved during the two successive seasons of 2016 and 2017. Results showed that increasing the rates of propolis extract as foliar application increased the growth parameters of the treated plants. The best result was obtained by the rate (8000 mg/L) as a foliar application in both seasons of the study. The same trend was also observed regarding all studied chemical constituents, i.e. chlorophyll a, b and total carotenoids concentration, anthocyanine, total carbohydrates, total and reducing sugars, total free amino acid, free proline, crude protein, total indoles, total phenols, N, P and K in leaves. Thus, the coincident application of propolis extract at (8000 mg/L) as foliar application ingredient is recommended for improving growth, yield and chemical composition of spinach plants and for overcoming the adverse effect of saline conditions.
Keywords
Spinach Plants (Spinacia Oleracea L.); Propolis Extract; Salinity; Growth; Yield; Chlorophyll; Carotenoids; Anthocyanin; Carbohydrates; Sugars; Free Amino Acid; Indoles; Phenols; Proline.
Cite this Article
El-Yazal, M., A., S. (2019), Impact of Propolis Extract as Foliar Spray On Growth, Yield and Some Chemical Composition of Spinach (Spinacia Oleracea L.) Plants Grown Under Calcareous Saline Soil. International Journal For Empirical Education and Research, 3(19), 1-14.
El-Yazal, M., A., S. (2019) "Impact of Propolis Extract as Foliar Spray On Growth, Yield and Some Chemical Composition of Spinach (Spinacia Oleracea L.) Plants Grown Under Calcareous Saline Soil", International Journal For Empirical Education and Research, 3(19), pp.1-14.
El-Yazal, M., A., S.. Impact of Propolis Extract as Foliar Spray On Growth, Yield and Some Chemical Composition of Spinach (Spinacia Oleracea L.) Plants Grown Under Calcareous Saline Soil. International Journal For Empirical Education and Research. 2019; 3(19): 1-14.
Appeared
Appeared
El-Yazal, M., A., S.. Impact of Propolis Extract as Foliar Spray On Growth, Yield and Some Chemical Composition of Spinach (Spinacia Oleracea L.) Plants Grown Under Calcareous Saline Soil. International Journal For Empirical Education and Research. 2019; 3(19): 1-14.
El-Yazal, M., A., S.. Impact of Propolis Extract as Foliar Spray On Growth, Yield and Some Chemical Composition of Spinach (Spinacia Oleracea L.) Plants Grown Under Calcareous Saline Soil. International Journal For Empirical Education and Research. 2019; 3(19): 1-14.
Reference
1. H. Biemond, H. Vos, P.C. Struik, Effects of nitrogen on accumulation and partitioning of dry matter and nitrogen vegetable, Netherland Journal of Agricultural Science. 44(1996)227-229.
2. E. Nishihara, M. Inoue, K. Kondo, K. Takahashi, N. Nakata, Spinach yield and nutritional quality affected by controlled soil water matric head, Agricultural Water Management. 51 (3) (2001)217-229.
3. S. Ors, D.L. Suarez, Spinach biomass yield and physiological response to interactive salinity and water stress, Agric. Water Manag.190(2017) 31–41.
4. S. Ors, D. L. Suarez, Salt tolerance of spinach as related to seasonal climate, Hort. Sci. (Prague) 43 (1) (2016) 33–41.
5. M. Najafi-Ghiri, T. Rahimi, Zinc Uptake by Spinach (Spinacia oleracea L.) as Affected by Zn Application Rate, Zeolite, and Vermicompost, Compost Science & Utilization, 24(2016) (3) 203-207.
6. J.F.S. Ferreira, D. Sandhu, X. Liu, J.J. Halvorson, Spinach (Spinacea oleracea L.) response to salinity: nutritional value, physiological parameters, antioxidant capacity, and gene expression, Agriculture. 8(10) (2018) 163; doi:10.3390/agriculture8100163
7. W.M. Semida, M. M. Rady, Presoaking application of propolis and maize grain extracts alleviates salinity stress in common bean (Phaseolus vulgaris L.), Scientia Horticulturae. 168(2014)210–217.
8. A.I. Rushdi, N. Adgaba, N.I.M. Bayaqoob, A. Al-Khazim, B.I.T. Simoneit, A.H. El-Mubarak, K.F. Al-Mutlaq, Characteristics and chemical compositions of propolis from Ethiopia, Springer Plus. 3(2014) 253. http://www.springerplus.com/content/3/1/253
9. S. Ramnath, S. Venkataramegowda, C. Singh, Chemical Composition of Bee Propolis Collected from Different Regions in India by GCMS Analysis, International Journal of Pharmacognosy and Phytochemistry. 30 (1) (2015) 1319-1328.
10. B.A.S. Machado, R.B.D. Silva,G.A. Barreto,S.S. Costa, D.F. da Silva,H.N. Brandão, J.L.C. da Rocha, O.A. Dellagostin, J.A.B. Henriques, M.A. Umsza-Guez, F.F. Padilha1, Chemical composition and biological activity of extracts obtained by supercritical extraction and ethanolic extraction of brown, green and red propolis derived from different geographic regions in Brazil, PLoS One. 11(1) (2016) e0145954
11. A.M. Saad, M.A. Ghareeb, M.S. Abdel-Aziz, H.M.F. Madkour, O.M. Khalaf, A.K. El-Ziaty, M. Abdel-Mogib, Chemical constituents and biological activities of different solvent extracts of Prosopis farcta growing in Egypt, Journal of Pharmacognosy and Phytotherapy. 9(5) (2017) 67-76.
12. A.A. Al-Ghamdi, N.I.M. Bayaqoob, A.I. Rushdi, Y. Attala, B.R.T. Simoneit, A.H. El-Mubarak, K.F. Al-Mutlaqe, Chemical compositions and characteristics of organic compounds in propolis from Yemen, Saudi Journal of Biological Sciences. 24(5) (2017) 1094-1103.
13. H.M. Fathy, A.S. Fatehe, M.H. Ahmed, M.G. Ghazy, A. Farid Badria, Chemical and biological diversity of propolis samples from Bulgaria, Libya, and Egypt, Journal of Apitherapy. 3 (2) (2018) 17–23.
14. E.M. El-Assiuty, Z. M. Fahmy, A.S. M. Ismael, F.M. Bekheet, propolis in controlling sorghum downy mildew and stimulating plant growth of maize, Egypt J. Appl. Sci. 15(12) (2000) 45-54.
15. M.M. Rady, Response of propolis extract-presoaking seeds of some crops to salt tolerance under different soil conditions, Ph.D. Thesis Fac. Agric. Fayoum, Cairo University (2002).
16. E.M.A. However, M. G. Dawood, Efficiency of propolis extract on faba bean plants and its role against nematode infection, Comm. Appl. Sci, Ghent University 74(2) (2009) 593-603.
17. A. Abou-Sreea, S. Mahfouz, R. M. Zewainy, Effectiveness of propolis aqueous extract on chemical constituents of Calendula Plants, International Journal of Pharmaceutical and Clinical Research. 9 (2) (2017) 137-143.
18. A. Klute, Methods of Soil Analysis Part 1, Physical and Mineralogical Methods, 2nd Edition. American Society of Agronomy, Medison, Wisconsin, U.S.A.1986.
19. A. I. Page, R. H. Miller, D. R. Keeny, “Methods of Soil Analysis”. Part 2: Chemical and Microbiological Properties. 2nd Ed (1982). Amer. Soc. Agron., Madison, Wisconsin, USA.
20. L. Vechet, Effect of propolis on some species of microorganisms and molds, In a remarkable hive product. Propolis 1978, pp.53-59. Bucharest, Romania: Apimondia Publishing house.
21. P. Walker, E. Crane, Constituents of propolis, Apidologie, 18(4) (1987) 327-334.
22. A.R. Wellburn, H. Lichtenthaler, Formulae and program to determine total carotenoids and chlorophyll a and b of leaf extracts different solvents. In Advances in Photosynthesis Research (Sybesma C.Ed.) Vol., II 1984, pp. 9-12. Martinus Nijhoff Dr. W. Junk Publishers, The Hague.
23. D. Herbert, P.J. Phipps, R.F. Strange, Determination of total carbohydrates. Methods in Microbial, 5 (B) (1971) 209-244.
24. A.O.A.C., Official Methods of Analysis of the Association of Official Agricultural Chemists, Sixteenth ed. (1995), Washington D.C., USA.
25. R. E. Hoagland, Effect of pyrophosphate on the metabolism of phenolic compounds. VI. Effect of glyphosate and glyphosate metabolites on phenylalanine ammonia lyase activity, growth, protein, and chlorophyll an anthocyanin levels in soybean seedlings, Weed Sci. 28(1980) 393.
26. J. Jayaraman, Laboratory Manual in Biochemistry. Willey Eastern limited, New York,1981, pp. 61-73.
27. P. Larson, A. Harbor, S. Klunsour, T. Aasheim, On the biogenesis of some indole compounds in Acetobacter xylimum, Physiol. Plant., (15) (1962) 552-565.
28. L.S. Bates, R.P. Waldren, I.D. Tears, Rapid determination of free proline for water stress studies plant and soil, 39(1973) 205-207.
29. K.A. Gomez, A.A. Gomez, Statistical Analysis Procedure of Agricultural Research, John Wiley and Sons, New York, 1983, pp. 25-30.
30. A. B. Nikolaev, Defining the bee town, In A remarkable hive product: Propolis. Scientific data and suggestions concerning its composition, properties and possible use in therapeutics. Apimondia Standing Commission on Beekeeping Technology and Equipment, Bucharest (1978). (c.f. A.G. Hegazi, Propolis an overview, International Symposium on Apitherapy, National Research Center, Cairo, Egypt, March 8-9th, 1997).
31. M.I. Salama, A.A. Elaidy, A. El-Sammak, A.M. Abou- Khashab, Leaf pigment and nutrient element content of Roumi Red grape nurslings as affected by salinity and some growth regulators, J. Agric. Rec. Tanta Univ., 18(2) (1992) 382-391.
32. H. M. El-Saht, Metribuzin herbicide-induced a defense mechanism in normal and NaCl- stressed castor bean and maize plants, Egypt. J. Hort. 28, (2) (2001) 277-290.
33. T. J. Flowers, P.P. Troke, A. R. Yeo, The mechanism of salt tolerance in halophytes, Ann. Rev. Plant Physiol., (28) (1977) 89-121.
34. V.S. Bankova, S.L. De Castro, M.C. Marucci, Propolis: recent advances in chemistry and plant origin, Apidologie 31(2000) 3–15.
35. M. A. EL-Tayeb, Effect of thiamin seed presoaking on the physiology of Sorghum Bicolor L. plants grown under salinity stress, Egypt, J. Bot., 35(2) (1995) 201-214.
36. P. Gopala Rao, C. Damodara Reddy, J. K. Ramaiah, Effect of B vitamins on the protein component of cluster bean Cyamopsis tetragonoloba L., Taub, Ann. Bot., (59) (1987) 281- 292.
37. R.M. Devlin, F. H. Witham, Plant physiology.4th Ed. CBS Publishers and Distributors, 485 Jain Bhawan, Bhola Nath Nagar, Shahdara, Delhi-110 032 (India), 1986, p. 443.
38. N. Das, M. Misra, A.N. Misra, Sodium chloride salt stress-induced a metabolic change in callus cultures of Pearl Millet (Pennisetum americanum L. Leek. ) free solute accumulation, J. Plant Physiol., (137) (1990) 244- 246.
39. S. Weidner, Role of gibberellins and cytokinins in the regulation of germination during development and ripening of Triticale caryopses L., Acta Societatis, Botanicorum Polonia, 53(2) (1984) 257-270.
40. L. Zhaoliang, S. Yueqing, S. Mingzhi, L. Zi, S. Yq, S. Mz, S. Yh, The effect of paclobutrazol on plant histology of some crops, Acta Agric Shanghai, 11(1995) 43- 47
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