Submission Open for IJEER Volume-3, Number-10, October 2019 | Submission Deadline- 20 October, 2019

International Journal for Empirical Education and Research

Changes in Metabolic Processes during Break Dormancy in Apple Buds under Foliar-Applied Garlic Extract

Author: Mohamed A. Seif El-Yazal | Published on: 2018-04-30 15:12:01   Page: 36-57   593

Abstract
The effect of garlic extract at different concentrations for reaching early break dormancy in buds of "Anna" apple (Malus sylvestris, Mill) trees and their effects on metabolic changes in the content of shoots from (water content %, total carbohydrates, total sugars, reducing sugars, anthocyanine, total free amino acids, free proline, total indoles and free phenoles) during their release from dormancy were investigated. The trees were grown in loamy sand soil, and sprayed with five treatments; 0, 5, 10, 15 and 20% garlic extract. Generally, it was found that all studied flowering parameters; date of flower buds break, percentage of bud’s break, fruit set, total number of fruits/tree, yield/tree (kg) and seasonal changes in some chemical constituents of shoots; water content %, total carbohydrates, total sugars, reducing sugars, anthocyanine, total free amino acids, free proline and total indoles were increased with the application of different treatments. The best results were obtained from the treatments of garlic extract at 15 % flowed by 20 %. On the contrary, the same treatments decreased free phenols in shoots as compared to the control. It could be recommended to use garlic extract at the rate of 15 % for improving bud break, growth, yield and chemical constituents of apple shoots

Keywords
Apple (Malus sylvestris, Mill); Dormancy; Garlic extract; Bud break; Flowering; Yield; Chemical constituents.

Cite this Article

×

Mohamed. A. (2018), Changes in Metabolic Processes during Break Dormancy in Apple Buds under Foliar-Applied Garlic Extract. International Journal For Empirical Education and Research, 2(9), 36-57.

  • Download Citation
  • ×

    Mohamed. A. (2018) "Changes in Metabolic Processes during Break Dormancy in Apple Buds under Foliar-Applied Garlic Extract", International Journal For Empirical Education and Research, 2(9), pp.36-57.

  • Download Citation
  • ×

    Mohamed. A.. Changes in Metabolic Processes during Break Dormancy in Apple Buds under Foliar-Applied Garlic Extract. International Journal For Empirical Education and Research. 2018; 2(9): 36-57.

  • Download Citation
  • ×

    Appeared

    ×

    Appeared

    ×

    Mohamed. A.. Changes in Metabolic Processes during Break Dormancy in Apple Buds under Foliar-Applied Garlic Extract. International Journal For Empirical Education and Research. 2018; 2(9): 36-57.

  • Download Citation
  • ×

    Mohamed. A.. Changes in Metabolic Processes during Break Dormancy in Apple Buds under Foliar-Applied Garlic Extract. International Journal For Empirical Education and Research. 2018; 2(9): 36-57.

  • Download Citation

  • Reference

    1. Abd El-Rzek E, Abd El-Migeed MM, Abdel-Hamid N (2011) Effect of Spraying Garlic Extract and Olive Oil on Flowering Behavior, Yield and Fruit Quality of ‘Canino’ Apricot Trees. American-Eurasian J Agric & Environ Sci 11: 776-781.
    2. Abou- Hussein, M R (1970) Effect of some agricultural procedures on flowering, fruit setting and yield of Cucurbitaceae. Ph.D.Thesis, Alexandria Univ.
    3. Ahmed MAM, Eman, AA, Abd El-Migeed MMM (2009) Effect of garlic extract and mineral oil spray on flowering, Harvesting time, yield and fruit quality of Peach trees c.v. 'Florida prince'. Eastern and Russian J Plant Sci and Biotechnol 3: 53-57.
    4. A.O.A.C. (1995) Official Methods of Analysis of the Association of Official Agricultural Chemists. 16th ed., Washington D.C., USA.
    5. Allona I, RamosA, Ibáñez C, ContrerasA, Casado R, Aragoncillo C (2010) Review. Molecular control of winter dormancy establishment in trees. Spanish Journal of Agricultural Research 6: 201-210.
    6. Bachelard EP, Wightman F (1973) Biochemical and physiological studies   on dormancy release in tree buds. Can J Bot 51:2315-2326.
    7. Baz AG (1984) Some physiological studies on dormancy in Mit- Ghamr peach cultivar. Aseasonal changes in native growth materials related to bud dormancy in peach. Annals of Agric Sci Moshtohor 21:465-479.
    8. Biasi LA, Lipski B, Silva ED, Oliveira OR, Sachi AT, Peressuti RA (2010) Lime sulphur mineral oil and garlic extract to suppress dormancy of kiwi. Revista de Ciencias Agroveterinarias 9:58-65.
    9. Borkowska B (1980) Releasing the single apple buds from dormancy under the influence of low temperature, BA and ABA. Fruit Sci Rep 7:174-183.
    10. Botelho RV, Muller MM (2007a) Evaluation of garlic extract on bud dormancy release of "Royal Gala" apple trees. Australian Journal of Experimental Agriculture 47:738-741.
    11. Botelho RV, Muller MM (2007b) Garlic extract as alternative on bud dormant break of apple trees cv. Fuji Kiku. Rev Bras Frutic 29: 37-41.
    12. Botelho RV, Pavanello AP, Pires EJP, Terra MM, Mullr MM (2007) Effect of chilling and garlic extract on bud dormancy release in Cabernet Sauvignon grapevine cuttings. Am J Enol Vitic 58: 402-404.
    13. Botelho RV, Maia AJ, Pires EJP, Terra MM (2009) Effect of garlic extract on bud break of grapevines and in vitro control of causal agent of antract (Elsinoe ampelina Shear). Rev Bras Frutic [online]31:96-102.
    14. Botelho RV, Pires EJ, Moura MF, Terra MM, Tecchio MA (2010) Garlic extract improves budbreak of the "Niagara " grapevines on sub-tropical regions. Ciencia Rural, Santa Maria 40:2282-2287.
    15. Chen L, Chen Q, Zhang Z, Wana X (2009) A novel colorimetric determination of free amino acids content in tea infusions with 2,4-dinitrofluorobenzene. Journal of Food Composition and Analysis 22:137–141.
    16. Corrales-Maldonado CC, Martinez-Tellez MA, Gardea AA, Orozco-Avitia A, Vargas-Arispuro I (2010) Organic alternative for breaking dormancy in "Table grapes" in hot regions. Amer J of Agric & Biol Sci 5:143-147.
    17. De-Kok LJ, Stulen I (1993) Role of glutathione in plants under oxidative stress. In "Sulfur Nutrition and Assimilation in Higher Plants" (L. J. De Kok, I. Stulen, H. Rennenberg,C. Brunold and W. E. Rauser, eds.), pp.125-138. SPB Academic Publishing the Netherlands.
    18. Dokoozlian NK, Williams LE, Neja RA (1998) Surfactants improve the response of grapevines to hydrogen cyanamide. Hort Science Alexandria 33:857-859.
    19. El-Mansy HI, Walker DR (1969) Seasonal fluctuation in El-berta peach flower bud during and after the termination of rest. J Amer Soc Hort Sci 94:298.
    20. Ennajeh M, Vadel AM, Khemira H, Ben Mimoun M, Hellali R (2006) Defense mechanisms against water deficit in two olive (Olea europaea L.) cultivars ‘Meski’ and ‘Chemlali’. J Hortic Sci Biotechnol 81: 99–104.
    21. Erdei L, Stuiver B, Kuiper PJ (1980) The effect of salinity on lipid composition and on activity of Ca2+ and Mg2+ stimulated ATPases in salt-sensitive and salt- tolerant Plantago species. Physiol Plant 49: 315-319.
    22. Eshghil S, Rahemil M, Karamil A (2010) Overcoming winter rest of grapevine grown in subtropical regions using dormancy-breaking agents. Iran Agric Rese 29:99-106.
    23. Galicia L, Nurit E, Rosales A, Palacios-Rojas A (2009) Laboratory protocols: Maize nutrition quality and plant tissue analysis laboratory. Mexioc, DF: CIMMYT.
    24. Gemma H (1995) Dormancy breaking in Japanese pears grown in a heated greenhouse. Acta Hort 395:57-67.
    25. George AP, Nissen RJ, Lioyed J, Richens K (1990) Factors affecting fruit quality of low chill stone fruit in subtropical Australia. Acta Hort279: 559-564.
    26. Gomez KA, Gomez AA (1984) Statistical Analysis Procedure of Agricultural Research. John Wiley and Sons, New York, pp. 25-30.
    27. Hill-Cottingham DG (1968) The effect of climate and time of application of fertilizer on the development and crop performance of fruit trees. In recent aspects of nitrogen metabolism in plants. Eds E J Hewitt and C V Cutting 234-253 Acdemic Press London and New York.
    28. Horbowicz M, Grzesiuk A, Debski H, Koczkodaj D, Saniewski M (2008) Methyl jasmonate inhibits anthocyanins synthesis in seedlings of common buckwheat (Fagopyrum esculentum Moench). Acta Biol Crac Ser Bot 50:71–78.
    29. Horbowicz M, Mioduszewska H, Koczkodaj D, Saniewski M (2009) The effect of methyl jasmonate and phenolic acids on growth of seedlings and accumulation of anthocyanins in common buckwheat (Fagopyrum esculentum Moench). Acta Agrobot 62:49–56.
    30. Judd MJ, Meyer DH, Meekings JS, Richardson AC, Walton EF (2010) An ftir study of the induction and release of kiwifruit buds from dormancy. J Sci Food Agric 90:1071-1080.
    31. Jayarman J (1981) Laboratory Manual in Biochemistry, Wiley Eastern Limited New York, pp 61-73.
    32. Kubota N, YamaneY, Toriu K, Kawazu K, Higuchi T, Nishimura S (1999) Identification of active substances in garlic responsible for breaking bud dormancy in grapevines. Journal of the Japanese Society for Horticultural Science 68: 1111-1117.
    33. Kuroi I (1974) Studies on the promotion of grape vines by the lime nitrogen treatment during the rest period. Memoirs of the Fac Of Agric Niigata Univ 12:1-17.
    34. Luna V, Soriano MD, Bottini R, Sheng C, Pharis RP (1993) Levels of endogenous gibberellins, abscisic acid, Indole 3 acetic acid and naringenin during dormancy of peach flower buds. Acta Hort 329:165 -267.
    35. Mancinelli AL (1984) Photoregulation of anthocyanin synthesis. VIII. Effects of light pretreatments. Plant Physiol 75:447–453.
    36. Marschner H (1995) Mineral Nutrients of Higher Plant, Second Edition. Academic Press Harcoust Brace and Company, Published New York.
    37. Miyazaki JH, Yang SF (1987) The methionine salvage pathway in relation to ethylene and polyamine synthesis. Physiol Plant 69: 366-370.
    38. O,kennedy BT,  Hennerty MJ, Titus JS (1975) Changes in the nitrogen reserves of apple shoots during the dormant season. J Hort Sci 50:321-329.
    39. Pinto M, Lira V, Ugalde H, Perez F (2007) Fisiologia de la latencia de las yemas de vid: hipótesis actuales. Santiago: Universidad de Chile p.16

      Availablefrom: http://agronomia.uchile.cl/extension/serviciosyproductos/gie/publicaciones. Accessed: Dec 24, 2007.

    1. Rao P, Pattabiraman TN (1989) Reevaluation of the phenolsulfuric acid reaction for the estimation of hexoses and pentoses. Anal Biochem 181: 18-22.
    2. Rennenberg H (1989) Synthesis and emission of hydrogensulfide by higher plants In 'Biogenic Sulfur in the Environment " (E. S. Saltzman and W. J. Cooper, eds.), pp. 44-57. ACS Symp. Series 3296, Washington, DC.
    3. Robert MD, Francis HW (1985) Plant Physiology, Fourth Edition.
    4. Sagi F, Garay AS (1961) The dependence of auction oxidase activity on the photo periodically conditioned phenol content of the leaves and their age in Lupinus albus. Hort Abs 31:3688.
    5. Schmidt A (1986) Regulation of sulfur metabolism in plants. Prog Bot 48: 133-150.
    6. Skene KG (1969) A comparison of the effects of cycocell and tipping on fruit set Vitis vinefra L. Aust J Bio Sci 22: 1305-1311.
    7. Snell FD, Snell CT (1967) Azo compounds, nitrogen-containing cycles, and so on In: Snell FD, Snell CT, authors. Colorimetric methods of analysis including photometric methods. Princeton: D Van Nostrand Co Inc pp 448-601.
    8. Snellings SL, Takenaka NE, Kim-Hayes Y, Miller DW (2003) Rapid colorimetric method to detect indole in shrimp with gas chromatography mass spectrometry confirmation. Journal of Food Science 68: 1548-1553.
    9. Stuiver CE, Kuiper PJ, Marschner H, Kylin A (1981) Effect of salinity and replacement of K+ by Na+ on lipid composition in two sugar beet inbred lines. Physiol Plant 52: 77-82.
    10. Subha-drabandhu S (1995) Induction of bud break in apple trees that eceived insufficient chilling by hydrogen cyanamide. Acta Hort409:171-178.
    11. Tromp J (1970) Storage and mobilization of nitrogenous compounds in apple trees with special reference to arginine. In physiology of trees crops., Eds. L.C. Luckwill and C.V. cutting, 14: 3-59. Academic Press, Londone and New York.
    12. Tunner JN (1972) Practical uses of gibberellins in Agriculture and Horticulture. Plant Protection 7:1
    13. Vargas-Arispuro I, Corrales-Maldonadoy C, Martínez-Téllez MA (2008). Compounds derived from garlic as bud induction agents in organic farming of Table grape. Chilean Journal of Agricultural Research 68(1):94-101.
    14. Wang SF, Clark CJ, Boldingh HL (1991) Changes in the activities of catalase, peroxidase and polyphenol oxidase in apple buds during bud break induced by thidiazurin. J of Plant Growth Regulation, 10:3-39.
    15. Whitworth JL, Young E (1992) Chilling unit accumulation and forcing effects on carbohydrates of young apple rootstocks. J Hort Sci67:225-230.
    16. Young E (1989) Cytokinin and soluble carbohydrates concentrations in xylem sap of apple during dormancy and bud break. J Amer Soc Hort Sci 114:297-300.

    [This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).]

    Author Details


    Mohamed A. Seif El-Yazal
    Botany Department
    Fayoum University
    mas04@fayoum.edu.eg